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Worthington組織消化指南
引言
組織消化酶主要用于組織培養(yǎng)和細胞生物學研究的原代細胞的分離和收集。盡管這些消化酶已經(jīng)普遍應用多年，但是對其組織消化和細胞收集的作用機制卻沒有全面的理解。 由此導致的結果便是選擇一種消化方法大多是隨機選擇或基于過去的經(jīng)驗，而不是在*理解消化原理的基礎上選擇的消化方案，以及如何根據(jù)具體實驗目的修正消化方案以獲得更好的實驗結果。
細胞分離程序的目的是zui大程度的獲得功能活性的單個細胞，而影響結果的原因有很多，包括（不限于）以下：
1、組織類型
2、物種來源
3、動物年齡
4、遺傳改造情況（如knockouts）
5、所用的消化培養(yǎng)液
6、所用的酶
7、消化酶中的雜質
8、所用消化酶的工作濃度
9、溫度
10、孵育時間
通常前四個原因沒辦法選擇，而剩下的原因一般由經(jīng)驗決定以獲取zui適實驗結果。
通過查閱文獻來選擇*組織消化酶和zui適消化條件的研究人員通常被互相矛盾的實驗數(shù)據(jù)搞得一頭霧水。這些巨大差異來源于組織胞外基質的復雜而動態(tài)的特性，以及過去常用的粗制、未提純的組織分離酶。另外，組織胞外基質由各種蛋白、糖蛋白、脂類和糖脂組成，這些成分的組成比例在不同物種、不同組織、甚至不同發(fā)育（年齡）之間存在明顯的差異，這些因素都極大的影響了消化方案的制定。目前通常使用的粗制酶，如鏈霉蛋白酶、NF1:250、以及含有幾種其他不同濃度蛋白酶的膠原酶、以及各種多糖酶、核酶和脂酶。
本指南總結了目前常用組織消化酶的zui適消化和細胞收集知識和方法，描述了標準的實驗步驟， 以便于制定一個*的細胞分離方案，同時列舉了一些相關的參考文獻。
下表列出多種組織的適宜消化酶（含參考文獻，以組織類型和物種分類，詳見鏈接）
脂肪組織 | 腎上腺組織 | 骨組織 | 腦組織 |
軟骨組織 | 結腸組織 | 內皮組織 | 上皮組織 |
眼 | 心臟 | 腸 | 腎 |
肝 | 肺 | 淋巴結 | 乳腺 |
其它組織 | 其他組織 | 肌組織 | 神經(jīng)組織 |
胰腺 | 腮腺 | 垂體 | 前列腺 |
生殖 | 動物鱗片 | 皮膚 | 脾臟 |
干細胞 | 胸腺 | 甲狀腺/甲狀旁腺 | 扁桃體 |
腫瘤 |
|
|
|
注：使用Worthington公司消化酶的文獻不限于上表鏈接中所列，一般而言，Worthington公司的組織消化酶可用于大多組織類型的消化。
心臟組織消化手冊
| 物種 | 詳細來源 | 細胞名稱 | 消化用酶 | 培養(yǎng)基 | 參考文獻 |
| 牛 | 牛 | Heart | Trypsin: 0.06% | 25mM HEPES buffered Locke's solution, CMF | Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and B綿羊 Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990 |
| (包括大鼠) | Adrenal chromaffin | ||||
| Paraneurons | |||||
| 犬 | 犬 | Cardiomyocytes | Collagenase Type 2: 150 u/ml | Tyrode's solution | Zhang,D., Wu, C., Qi, X., Meijering, R., Hoogstra-Berends, F., Tadevosyan, A., Cubukcuoglu, et al: Activation of Histone Deacetylase-6 Induces Contractile Dysfunction Through Derailment of alpha-tubulin Proteostasis in Experimental and Human Atrial Fibrillation., Circulation 129, 346-58, 2014 |
| 犬 | 犬, adult | Atrial and Ventricular Myocytes | Collagenase Type 2: 0.065% | See reference | Bonilla, I., Sridhar, A., Nishijima, Y., Gyorke, S., Cardounel, A. and Carnes, C.: Differential Effects of the Peroxynitrite Donor, SIN-1, on Atrial and Ventricular Myocyte Electrophysiology., J Cardiovasc Pharmacol 61, 401-7, 2013 |
| 犬 | 犬 | Cardiomyocytes | Collagenase Type 2: 300 u/ml | Tyrode's solution | Xi, Y., Wu, G., Ai, T., Cheng, N., Kalisnik, J., Sun, J., Abbasi, S., Yang, D., Fan, C., Yuan, X., Wang, S. et al: Ionic Mechanisms Underlying the Effects of Vasoactive Intestinal Polypeptide on Canine Atrial Myocardium., Circ Arrhythm Electrophysiol 6, 976-83, 2013 |
| Protease: 0.03% | |||||
| 犬 | 犬 | Atrial Myocytes | Collagenase Type 2: 0.03% | Tyrode's solution | Gan, T., Qiao, W., Xu, G., Zhou, X., Tang, B., Song, J., Li, Y., Zhang, J., Li, F., Mao, T. and Jiang, T.: Aging-Associated Changes in L-type Calcium Channels in the Left Atria of Dogs., Exp Ther Med 6, 919-924, 2013 |
| 犬 | 犬 | Cardiomyocytes | Collagenase Type 2: 60 u/ml | See reference | Harleton, E., Besana, A., Comas, G., Danilo, P., Rosen, T., Argenziano, M., Rosen, M., Robinson, R. and Feinmark, S.: Ability to Induce Atrial Fibrillation in the Peri-Operative Period is Associated with Phosphorylation-Dependent Inhibition of TWIK Protein-Related Acid-Sensitive Potassium Channel 1 (TASK-1)., J Biol Chem 288, 2829-38, 2013 |
| Protease XIV: 0.5 u/ml | |||||
| 犬 | 犬 | Atrial and Ventricular Myocytes | Collagenase Type 2: 0.05-0.08% | See reference | Zhang, H., Silva, J., Lin, Y., Verbsky, J., Lee, U., Kanter, E., Yamada, K., Schuessler, R. and Nichols, C.: Heterogeneity and Function of K(ATP) Channels in Canine Hearts., Heart Rhythm 10, 1576-83, 2013 |
| 犬 | 犬, adult | Cardiomyocytes | Collagenase Type 2: 0.05% | HEPES | Calloe, K., Nof, E., Jespersen, T., Di Diego, J., Chlus, N., Olesen, S.? Antzelevitch, C. and Cordeiro, J.: Comparison of the Effects of a Transient Outward Potassium Channel Activator on Currents Recorded from Atrial and Ventricular Cardiomyocytes., J Cardiovasc Electrophysiol 22, 1057-66, 2011 |
| Protease Type XIV: 0.01% | |||||
| 犬 | 犬, 20-25 kg | Cardiomyocytes | Collagenase Type 2: 0.05% | Tyrode's solution | Burashnikov, A., Zygmunt, A., Di Diego, J., Linhardt, G., Carlsson, L. and Antzelevitch, C.: AZD1305 Exerts Atrial Predominant Electrophysiological Actions and is Effective in Suppressing Atrial Fibrillation and Preventing its Reinduction in the Dog., J Cardiovasc Pharmacol 56, 80-90, 2010 |
| 犬 | 犬, adult | Cardiomyocytes | Collagenase Type 2: 0.05% | M199 | Gavi, S., Yin, D., Shumay, E., Wang, H. and Malbon, C.: Insulin-Like Growth Factor-I Provokes Functional Antagonism and Internalization of Beta1-Adrenergic Receptors., Endocrinology 148, 2653-62, 2007 |
| Protease: 0.008% | |||||
| 犬 | 犬 | Atrial and Ventricular myocytes | Collagenase Type 2: 0.065% | Tyrode's solution | Schotten, U., de Haan, S., Verheule, S., Harks, E., Frechen, D., Bodewig, E., Greiser, M., Ram, R., Maessen, J., Kelm, M., Allessie, M. and Van Wagoner, D.: Blockade of Atrial-Specific K+-currents Increases Atrial but not Ventricular Contractility by Enhancing Reverse Mode Na+/Ca2+-Exchange.,Cardiovasc Res 73, 37-47, 2007 |
| 犬 | 犬 | Atrial myocytes | Collagenase Type 2: 0.013% | HEPES | Baba, S., Dun, W., Hirose, M. and Boyden, P.: Sodium Current Function in Adult and Aged Canine Atrial Cells., Am J Physiol Heart Circ Physiol 291, H756-61, 2006 |
| 犬 | 犬, 20-30 kg | Cardiomyocytes | Collagenase Type 2: 110 u/ml | Tyrode's solution | Sun, H., Chartier, D., Leblanc, N. and Nat, S.: Intracellular Calcium Changes and Tachycardia-Induced Contractile Dysfunction in Canine Atrial Myocytes., Cardiovasc Res 49, 751-61, 2001 |
| 犬 | 犬 | Ventricular and Atrial Myocytes | Collagenase Type 4: 0.04% | HEPES/Tyrode's | Gintant, G.: Two Components of Delayed Rectifier Current in Canine Atrium and Ventricle. Does IKs Play a Role in the Reverse Rate Dependence of Class III Agents?, Circ Res 78, 26-37, 1996 |
| Collagenase Type 2: 125 u/ml | |||||
| 犬 | Dog, mongrel, adult | Myocytes | Hyaluronidase: 0.1% | Joklik's MEM | Spanier, A. and Weglicki, W.: Ca2+-Tolerant Adult Canine Myocytes: Preparation and Response to Anoxia/Acidosis, Am J Physiol 243, H448, 1982 |
| 雞 | 雞, embryonic | Cardiomyocytes | Trypsin: 0.13% | HBSS | Blech-Hermoni, Y. and Ladd, A.: Identification of Transcripts Regulated by CUG-BP, Elav-like Family Member 1 (CELF1) in Primary Embryonic Cardiomyocytes by RNA-seq., Genom Data 6, 74-76, 2015 |
| Collagenase Type 2: 0.13% | |||||
| Deoxyribonuclease I: 0.033% | |||||
| 雞 | 雞 embryos, 9-11 day incubated | Cardiomyocytes | Trypsin: 0.25% | PBS | Eschenhagen, T., Fink, C., Remmers, U., Scholz, H., Wattchow, J., Weil, J., et al.: Three-Dimensional Reconstitution of Embryonic Cardiomyocytes in a Collagen Matrix: A New Heart Muscle Model System, FASEB J 11, 683, 1997 |
| 雞 | 小雞, embryos, 10-11 day | Cardiomyocytes | Trypsin: 0.17% | HBSS, CMF | Wang, S., Greaser, M.L., Schultz, E., Bulinski, J.C., Lin, J.J., and Lessard, J.L.: Studies on Cardiac Myofibrillogenesis With Antibodies to Titin, Actin, Tropomyosin, and Myosin, J Cell Biol 107, 1075, 1988 |
| 雞 | 小雞, 10 day | Ventricular | Trypsin: 0.025% | HBSS, CMF | Kim, D., Okada, A., and Smith, T.W.: Control of Cytosolic Calcium Activity During Low Sodium Exposure in Cultured Chick Heart Cells, Circ Res 61, 29, 1987 |
| 雞 | 小雞, embryo, 11 day | Cardiomyocytes | Trypsin: 0.05% | HBSS, CMF | Jacob, R., Lieberman, M., Murphy, E., and Piwnica-Worms, D.: Effect of Sodium-Potassium Pump Inhibition and Low Sodium on Membrane Potential in Cultured Embryonic Chick Heart Cells, J Physiol 387, 549, 1987 |
| 雞 | 小雞, embryonic, 11-12-day-old | Myocytes | Trypsin: 0.025% | CMF solution | Murphy, E., Aiton, J., Russell, C., and Lieberman, M.: Calcium Elevation in Cultured Heart Cells: Its Role in Cell Injury, Am J Physiol Cell Physiol 245 (14), C316, 1983 |
| 雞 | 小雞, 8 day embryo | Myocytes | Trypsin NF 1:250: 0.25% | Rinaldini's buffer solution, CF | Gross, W., Schopf-Ebner, E., and Bucher, O.: Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells, Exp Cell Res 53, 1, 1968 |
| (also rat, neonate and mouse) | |||||
| 雞 | Hen, White Leghorn | Heart | Trypsin: 0.025% | Medium 199 | Dehann, R.: Regulation of Spontaneous Activity and Growth of Embryonic Chick Heart Cells in Tissue Culture, Dev Biol 16, 216, 1967 |
| 雞 | 小雞embryos, 5 day | Heart | Trypsin: 3.0% | Tyrode's solution, CMF | Steinberg, M.: "ECM": Its Nature, Origin, And Function in Cell Aggregation, Exp Cell Res 30, 257, 1963 |
| Liver | |||||
| 貓科動物 | 貓, mongrel, adult, 1.8-2.8 Kg | Myocytes | Collagenase Type 2: 0.12% | Kreb's Henseleit, CF | Silver, L., Hemwall, E., Marino, T., and Houser, S.: Isolation and Morphology of Calcium-Tolerant Feline Ventricular Myocytes, Am J Physiol 245, H891, 1983 |
| Ventricular | |||||
| 魚 | 魚, 斑馬魚 | Cardiomyocytes | Collagenase Type 2: 0.5% | MEM | Sander, V., Sune, G., Jopling, C., Morera, C. and Belmonte J.: Isolation and In Vitro Culture of Primary Cardiomyocytes from Adult Zebrafish Hearts., Nat Protoc 8, 800-9, 2013 |
| Collagenase Type 4: 0.5% | |||||
| 青蛙 | 青蛙 (Rana esculenta) | Myocytes | Trypsin: 0.04% | CF Ringer | Arrio-Dupont, M., and de Nay, D.: High Yield Preparation of Calcium-Tolerant Myocytes From Frog Ventricles, Biol Cell 54, 164, 1985 |
| 豚鼠 | Guinea pig, adult | Cardiomyocytes | Collagenase Type 2: 100 u/ml | M-199 | Zorn-Pauly K, Schaffer P, Pelzmann B, Bernhart E, Lang P, and Koidl B: L-type and T-type Ca2+ current in cultured ventricular guinea pig myocytes,Physiol Res 53(4), 369, 2004 |
| 豚鼠 | 豚鼠 | Cardiomyocytes | Collagenase Type 2: 100-200 u/ml | See reference | Dhamoon, A, Pandit, S., Sarmast, F., Parisian, K., Guha, P., Li, Y., Bagwe, S., Taffet, S. and Anumonwo, J.: Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current.,Circ Res 94, 1332-9, 2004 |
| 豚鼠 | Guinea pig, female, 300-350g | Coronary endothelial | Collagenase Type 2: 0.1% | see reference | Buxton I L, Kaiser R A, Oxhorn B C, Cheek D J: Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium, Am J Physiol Heart Circ Physiol 281, H1657-66, 2001 |
| 豚鼠 | Guinea pig, 250-450 g | Endothelial | Collagenase Type 2: 0.15% | Perfusing solution | Preisig-Muller, R., Mederos Y Schnitzler, M., Derst, C., and Daut J.:Separation of Cardiomyocytes and Coronary Endothelial Cells for Cell-Specific RT-PCR, Am J Physiol 277, H413, 1999 |
| (see reference) | |||||
| 豚鼠 | 豚鼠, male, 450-600 g | Myocytes | Hyaluronidase: 0.02% | Krebs Henseleit bicarbonate buffer | Stemmer, P., Akera,T., Brody, T., Rardon, D., and Watanabe, A.: Isolation and Enrichment of Ca2+-Tolerant Myocytes for Biochemical Experiments from Guinea-Pig Heart, Life Sci 44, 1231, 1989 |
| 豚鼠 | 豚鼠 | Cardiomyocytes | Collagenase: 0.04% | Tyrode solution, CF | Ishihara, K., Mitsuiye, T., Noma, A., and Takano, M.: The Mg2+ Block and Intrinsic Gating Underlying Inward Rectification of the K+ Current in Guinea-Pig Cardiac Myocytes, J Physiol 419, 297, 1989 |
| 豚鼠 | 豚鼠 | Myocytes | Hyaluronidase: 0.10% | Bicarbonate buffer, CF | Bridge, J., Spitzer, K., and Ershler, P.: Relaxation of Isolated Ventricular Cardiomyocytes by a Voltage-Dependent, Science 241, 823, 1988 |
| 人類 | 人類 | Cardiac | Collagenase: 0.045% | DPBS | Holt-Casper, D., Theisen, J., Moreno, A., Warren, M., Silva, F., Grainger, D., Bull, D. and Pa, A.: Novel Xeno-free Human Heart Matrix-Derived Three-Dimensional Scaffolds., J Transl Med 13, 194, 2015 |
| Pancreatin: 0.1% | |||||
| 人類 | 人類 | Cardiac stem | Collagenase Type 2: 0.25% | Joklik modified Eagle抯 | Avolio, E., Gianfranceschi, G., Cesselli, D., Caragnano, A., Athanasakis, E., Katare, R., Meloni, M., Palma, A., Barchiesi, A., Vascotto, C., Toffoletto, B., Mazzega, E., Finato, N., Aresu, G. and Livi, U.: Ex vivo Molecular Rejuvenation Improves the Therapeutic Activity of Senescent Human Cardiac Stem Cells in a Mouse Model of Myocardial Infarction., Stem Cells 32, 2373, 2014 |
| 人類 | 人類 | Atrial myocytes | Collagenase Type 1: 286 u/ml | see references | Voigt, N., Zhou, X. and Dobrev, D.: Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents., J Vis Exp , e50235, 2013 |
| Protease: 5 u/ml | |||||
| 人類 | 人類 | Myofibers | Collagenase Type 1: 0.3% | see reference | Anderson, E., Rodriguez, E., Anderson, C., Thayne, K., Chitwood, W. and Kypson, A.: Increased Propensity for Cell Death in Diabetic Human Heart is Mediated by Mitochondrial-Dependent Pathways., Am J Physiol Heart Circ Physiol 300, H118, 2011 |
| 人類 | 人類 | Atrial cardiomyocytes | Collagenase Type 1: 286 u/ml | see reference | Voigt, N., Makary, S., Nat, S. and Dobrev, D.: Voltage-Clamp-Based Methods for the Detection of Constitutively Active Acetylcholine-Gated I(K,ACh) Channels in the Diseased Heart., Methods Enzymol 484, 653, 2010 |
| Protease: 5 u/ml | |||||
| 人類 | 人類 | Coronary artery smooth muscle | Collagenase Type 2: 0.1% | DMEM | Jensen, B., Swigart, P., Laden, M., DeMarco, T., Hoopes, C. and Simpson, P.: The Alpha-1D Is the Predominant Alpha-1-Adrenergic Receptor Subtype in Human Epicardial Coronary Arteries., J Am Coll Cardiol 54, 1137, 2009 |
| Elastase: 0.05% | |||||
| 人類 | 人類 | Smooth muscle aortic | Collagenase Type 1: 0.2% | DMEM | Mathew, S., Tustison, K., Sugatani, T., Chaudhary, L., Rifas, L. and Hruska, K.: The Mechanism of Phosphorus as a Cardiovascular Risk Factor in CKD., J Am Soc Nephrol 19, 1092, 2008 |
| Collagenase Type 2: 0.1% | |||||
| Elastase: 0.025% | |||||
| 人類 | 人類 (n=16, age: 60 &plusmin;3 years) | Myocardial | Hyaluronidase: 0.05% | Medium 199 | Mukerjee, R., Multani, M., Sample, J., Dowdy, K., Zellner, J., Hoover, D., and Spinale, F.: Effects of adrenomedullin on human myocyte contractile function and beta-adrenergic response, J Cardiovasc Pharmacol 7 (4), 235, 2002 |
| 人類 | 人類 | Cardiomyocytes | Collagenase Type 2: 0.05% | HEPES solution | Todor Anastassia, Sharov Victor G, Tanhehco Elaine J, Silverman Norman, Bernabei Alvise, Sabbah Hani N: Hypoxia-induced cleavage of caspase-3 and DFF45/ICAD in human failed cardiomyocytes, Am J Physiol Heart Circ Physiol 283, H990-5, 2002 |
| Collagenase Type 1: 0.025% | |||||
| Protease XIV: 0.013% | |||||
| 人類 | 人類 | Heart | Collagenase Type 2: 200 u/ml | Tyrode's solution | Hoppe, U., Jansen, E., Sudkamp, M., and Beukelmann, D.:Hyperpolarization-Activated Inward Current in Ventricular Myocytes From Normal and Failing Human Hearts, Circulation 97, 55, 1998 |
| Myocytes | |||||
| 人類 | 人類 | Atrial myocytes | Collagenase Type 2: 0.1% | see reference | Van Wagoner, D., Pond, A., McCarthy, P., Trimmer, J. and Nerbonne, J.:Outward K+ Current Densities and Kv1.5 Expression are Reduced in Chronic Human Atrial Fibrillation., Circ Res 80, 772-81, 1997 |
| Protease Type XIV: 0.04% | |||||
| 人類 | 人類, fetal, 14.5 week gestation | Myocytes | Hyaluronidase: 0.1% | HBSS with Calcium | Goldman, B., and Wurzel, J.: Effects of Subc*tion and Culture Medium on Differentiation of Human Fetal Cardiac Myocytes, In Vitro Cell Dev Biol 28, 109, 1992 |
| 人類 | 人類, both sexes, 18-81 years | Myocardial | Collagenase: 0.14% | HBSS, CMF | Smith, D., Glover, J., Townsend, L., and Maupin, E.: A Method for the Harvest, Culture, and Characterization of Human Adult Atrial Myocardial Cells: Correlation with Age of Donor, In Vitro Cell Dev Biol 27, 914, 1991 |
| Atrial | |||||
| 人類 | 人類, adult | Myocytes | Trypsin: 0.25% | Joklik's MEM | Bugaisky, L.B.: Biology of Isolated Adult Cardiac Myocytes,Isolation and Culture of Human Adult Cardiac Myocytes,(Clark,Decker,Borg,eds), Elsevier Science Publishing Co., Inc., , 1988 |
| 人類 | 人類 | Thoracic aorta | Collagenase: 0.2% | Phosphate buffer w/NaCl | Hassler, O., Wiren, M., and Herbertsson, S.: The Elastic Coat of the Arterial Wall Studied with the Aid of Collagenase, Acta Pathol Microbiol Scand 57, 15, 1963 |
| 無脊椎動物 | E. cirhosa章魚, 260-352 克 | Systemic heart cardiomyocytes | Collagenase: 0.025% | see reference | Altimiras J., Hove-Madsen L., and Gesser H.: Ca(2+) Uptake in the Sarcoplasmic Reticulum from the Systemic Heart of Octopod Cephalopods, J Exp Biol 202, 2531, 1999 |
| Trypsin: 0.02% | |||||
| 小鼠 | 小鼠, 15-30 gm | Ventricular myocytes | Collagenase Type 2: 0.12% | see reference | Henn, M., Janjua, M., Kanter, E., Makepeace, C., Schuessler, R., Nichols, C. and Lawton, J.: Adenosine Triphosphate-Sensitive Potassium Channel Kir Subunits Implicated in Cardioprotection by Diazoxide., J Am Heart Assoc 4, , 2015 |
| 小鼠 | 小鼠, 3-4 week | Cardiac progenitor | Collagenase Type 1: 0.1% | DMEM | Wang, H., Chen, H., Feng, B., Wang, X., He, X., Hu, R., Yin, M., Wang, W., Fu, W. and Xu, Z.: Isolation and Characterization of a Sca-1+/CD31- Progenitor Cell Lineage Derived From Mouse Heart Tissue., BMC Biotech4, 75, 2014 |
| 小鼠 | 小鼠, adult | Cardiac Progenitor | Collagenase Type 2: 600 u/ml | MEM | Santos Nascimento, D, Mosqueira, D, Sousa, L, Teixeira, M, Filipe, M, Resende, T, Araujo, A, Valente, M, Almeida, J, Martins, J, Santos, J, Barcia, R, Cruz, P, Cruz, H and Pinto-d-O, P: Human Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Attenuate Remodeling after Myocardial Infarction by Proangiogenic, Antiapoptotic, and Endogenous Cell-Activation Mechanisms., Stem Cell Res Ther 5, 5, 2014 |
| Deoxyribonuclease I: 60 u/ml | |||||
| 小鼠 | 小鼠 | Cardiac progenitor | Collagenase Type 2: 0.1% | HBSS | Zafiriou, M., Noack, C., Unsold, B., Didie, M., Pavlova, E., Fischer, H., Reichardt, H., Bergmann, M., El-Armouche, A., Zimmermann, W., Zelarayan, L.: Erythropoietin Responsive Cardiomyogenic Cells Contribute to Heart Repair Post Myocardial Infarction., Stem Cells 32, 2480, 2014 |
| Neutral Protease: 2.4 u/ml | |||||
| 小鼠 | 小鼠, adult | Cardiomyocytes | Collagenase Type 2: 0.08% | see reference | Kohncke, C. Lisewski, U., Schleussner, L., Gaertner, C., Reichert, S. and Roepke, T.: Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes., J Vis Exp 73, e50145, 2013 |
| 小鼠 | 小鼠 | Cardiomyocytes | Collagenase Type 2: 150 u/ml | MEM | Hennessey, J., Wei, E. and Pitt, G.: Fibroblast Growth Factor Homologous Factors Modulate Cardiac Calcium Channels., Circ Res 113, 381-8, 2013 |
| 小鼠 | 小鼠 | Cardiomyocytes | Collagenase Type 2: 360 u/ml | L-15 | Touchberry, C., Green, T., Tchikrizov, V., Mannix, J., Mao, T., Carney, B., Girgis, M., Vincent, R., Wetmore, L., Dawn, B., Bonewald, L., Stubbs, J. and Wacker, M.: FGF23 is a Novel Regulator of Intracellular Calcium and Cardiac Contractility in Addition to Cardiac Hypertrophy., Am J Physiol Endocrinol Metab 304, E863, 2013 |
| Papain: 0.4 u/ml | |||||
| Deoxyribonuclease I: 40 u/ml | |||||
| 小鼠 | 小鼠, 2-6 month | Sinoatrial node | Collagenase Type 2: 229 u/ml | Tyrode's solution | Chris, C., Cardona, N., Mesirca, P., Herrmann, S., Hofmann, F., Striessnig, J., Ludwig, A., Mangoni, M. and Lee, A.: Distinct Localization and Modulation of Cav1.2 and Cav1.3 L-type Ca2+ Channels in Mouse Sinoatrial Node., J Physiol 590, 6327, 2012 |
| Elastase: 1.9 u/ml | |||||
| Protease Type XIV: 0.9 u/ml | |||||
| 小鼠 | 小鼠, male, 8-25 week | Cardiomyocytes | Collagenase Type 2: 59 u/ml | MEM | Carley, A. and Kleinfeld, A.: Fatty Acid (FFA) Transport in Cardiomyocytes Revealed by Imaging Unbound FFA is Mediated by an FFA Pump Modulated by the CD36 Protein., J Biol Chem 286, 4589-97, 2011 |
| 小鼠 | 小鼠 | Aortic adventitial leukocytes | Collagenase Type 2: 300 u/ml | PBS | Butcher, M., Herre, M., Ley, K. and Galkina, E.: Flow Cytometry Analysis of Immune Cells Within Murine Aortas., J Vis Exp 53, e2828, 2011 |
| Elastase: 5.6 u/ml | |||||
| 小鼠 | 小鼠, adult | Cardiomyocytes | Collagenase Type 1: 0.1-0.4% | see reference | Flynn, J., Santana, L. and Melov, S.: Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes., J Vis Exp 58, e3302, 2011 |
| Protease Type XIV: 0.004-.02% | |||||
| 小鼠 | 小鼠, male, 6 week | Coronary endothelial cells | Collagenase Type 2: 0.1% | M199 | Makino, A., Suarez, J., Wang, H., Belke, D., Scott, B. and Dillmann, W.:Thyroid Hormone Receptor-beta is Associated With Coronary Angiogenesis During Pathological Cardiac Hypertrophy., Endocrinology 150, 2008, 2009 |
| Neutral Protease: 0.6 u/ml | |||||
| 小鼠 | 小鼠, adult | Ventricular myoctes | Collagenase: 0.1% | M199 | Zhang, Y, Kanter, E., Laing, J., Aprhys, C., Johns, D., Kardami, E. and Yamada, K.: Connexin43 Expression Levels Influence Intercellular Coupling and Cell Proliferation of Native Murine Cardiac Fibroblasts., Cell Commun Adhes 15, 289, 2008 |
| 小鼠 | 小鼠, adult, 9-13 week | Cardiomyocytes | Collagenase Type 2: 620 u/ml | Myocyte buffer see reference | Kabaeva, Z., Zhao, M. and Michele, D.: Blebbistatin Extends Culture Life of Adult Mouse Cardiac Myocytes and Allows Efficient and Stable Transgene Expression., Am J Physiol Heart Circ Physiol 294, H1667-74, 2008 |
| Protease XIV: 0.104 u/ml | |||||
| Deoxyribonuclease I: 0.0015% | |||||
| 小鼠 | 小鼠, adult | Cardiomyocytes | Collagenase: see reference | HBSS | O'Connell Timothy D, Rodrigo Manoj C, Simpson Paul C: Isolation and culture of adult mouse cardiac myocytes, Methods Mol Biol 357, 271-96, 2007 |
| 小鼠 | 小鼠 | Smooth muscle cells | Collagenase Type 2: 300 u/ml | F10 Ham | Verheye, S., Martinet, W., Kockx, M., Knaapen, M., Salu, K., Timmermans, J., Ellis, J., Kilpatrick, D., DeMayer, G.: Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy, J Am Coll Cardiol49, 706, 2007 |
| Elastase: 5 u/ml | |||||
| 小鼠 | 小鼠, adult, 6-12 week | Cardiomyocytes | Collagenase Type 2: 0.1% | see reference | Shioya, T: A Simple Technique for Isolating Healthy Heart Cells from Mouse Models., J Physiol Sci 57, 327-35, 2007 |
| Trypsin: 0.006% | |||||
| Protease XIV: 0.006% | |||||
| 小鼠 | 小鼠 | Ventricular myocytes and mesenteric arterial SMC | Collagenase Type 2: 0.06% | Krebs-Ringer | Lu Tong, Ye Dan, Wang Xiaoli, Seubert John M, Graves Joan P, Bradbury J Alyce, Zeldin Darryl C, Lee Hon-Chi: Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms, J Physiol 575, 627-44, 2006 |
| Papain: 0.175% | |||||
| 小鼠 | 小鼠 | Cardiomyocytes | Collagenase Type 1: 0.17% | PBS | Chen, H., Yong, W., Ren, S., Shen, W., He, Y., Cox, K., Zhu, W., Li, W., Soonpaa, M., Payne, RM, Franco, D., Field, L., Rosen, V., Wang, Y. and Shou, W.: Overexpression of Bone Morphogenetic Protein 10 in Myocardium Disrupts Cardiac Postnatal Hypertrophic Growth., J Biol Chem 281, 27481, 2006 |
| 小鼠 | 小鼠, neonatal | Cardiomyoctes | Collagenase Type 1: 150 u/ml | M199 | Takahashi N, Wang X, Tanabe S, Uramoto H, Jishage K, Uchida S, Sasaki S, Okada Y.: ClC-3-independent sensitivity of apoptosis to Cl- channel blockers in mouse cardiomyocytes, Cell Physiol Biochem 15, 263, 2005 |
| Trypsin: 0.01% | |||||
| 小鼠 | 小鼠, 1 day | Cardiomyoctes | NCIS kit: per instructions | EBSS | Potts, Malia B., Vaughn, Allyson E., McDonough, Holly, Patterson, Cam, and Deshmukh, Mohanish: Reduced Apaf-1 levels in cardiomyocytes engage strict regulation of apoptosis by endogenous XIAP, J Cell Biol 171, 925, 2005 |
| 小鼠 | 小鼠 | Aortic endothelial | Collagenase Type 2: 0.2% | DMEM | Kobayashi, M., Inoue, K., Warabi, E., Minami, T. and Kodama, T.: A Simple Method of Isolating Mouse Aortic Endothelial Cells., J Atheroscler Thromb 12, 138, 2005 |
| 小鼠 | 小鼠 | Smooth muscle cells | Collagenase Type 1: 0.1% | DMEM | Fukumoto Yoshihiro, Deguchi Jun-o, Libby Peter, Rabkin-Aikawa Elena, Sakata Yasuhiko, Chin Michael T, Hill Christopher C, Lawler Patrick R, Varo Nerea, Schoen Frederick J, Krane Stephen M, Aikawa Masanori:Genetically determined resistance to collagenase action augments interstitial collagen accumulation in atherosclerotic plaques, Circulation 110, 1953-9, 2004 |
| Elastase: 0.0125% | |||||
| 小鼠 | 小鼠, female, 14-18 g | Cardiomyocytes | Collagenase Type 2: 0.02% | see reference | Zhang Sui, Wang Dachun, Estrov Zeev, Raj Sean, Willerson James T, Yeh Edward T H: Both cell fusion and transdifferentiation account for the transformation of human peripheral blood CD34-positive cells into cardiomyocytes in vivo, Circulation 110, 3803-7, 2004 |
| Elastase: 0.03% | |||||
| Pancreatin: 0.06% | |||||
| 小鼠 | 小鼠, 6-8 week | Vascular smooth muscle | Papain: 10 u/ml | EBSS | Qian, Q., Hunter, L., Li, M., Marin-Padilla, M., Prakash, Y., Somlo, S., Harris, P., Torres, V., and Sieck, G.: Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells, Hum Mol Genet 12(15), 1875, 2003 |
| Elastase: .005% | |||||
| Collagenase: 0.05% | |||||
| Deoxyribonuclease I: 1000 u/ml | |||||
| PDS kit: with modifications | |||||
| 小鼠 | 小鼠 | Cardiomyocytes | Collagenase Type 2: 0.2% | DMEM/F-12 | Rybkin, I., Markham, D., Yan, Z., Bassel-Duby, R., Williams, R., and Olson, E.: Conditional Expression of SV40 T-antigen in Mouse Cardiomyocytes Facilitates an Inducible Switch from Proliferation to Differentiation, J Biol Chem 278(18), 15927, 2003 |
| Pancreatin: 0.06% | |||||
| 小鼠 | 小鼠, neonatal 2-4 day | Atrial myocytes | NCIS kit: with modifications see reference | L-15 | Bettahi, I,, Marker, C., Roman, M., and Wickman, K.: Contribution of the Kir3.1 Subunit to the Muscarinic-gated Atrial Potassium Channel IKACh, J Biol Chem 277, 48282, 2002 |
| 小鼠 | 小鼠, neonatal and adult | Cardiomyocytes | Collagenase Type 2: 0.03-0.2% | Joklik's MEM | Schreiber, K., Paquet, L., Allen, B., and Rindt, H.: Protein Kinase C Isoform Expression and Activity in the Mouse Heart, Am J Physiol Heart Circ Physiol281(5), H2062, 2001 |
| 小鼠 | 小鼠, NTg and Tg, 8 - 12 week | Myocytes | Collagenase Type 2: 150 u/ml | Joklik's MEM | Nelson, D., Setser, E., Hall, D., Schwartz, S., Hewitt, T., Klevitsky, R., Osinska, H., Bellgrau, D., Duke, R., and Robbins, J.: Proinflammatory Consequences of Transgenic Fas Ligand Expression in the Heart, J Clin Invest 105 (9), 1199, 2000 |
| 小鼠 | 小鼠, 1-6 day | Cardiomyocytes | Collagenase Type 2: 0.1% | PBS | Watzka SB, Lucien J, Shimada M, Edwards V, Yeger H, Hannigan G, and Coles JG.: Selection of viable cardiomyocytes for cell transplantation using three-dimensional tissue culture, Transplantation 70, 1310, 2000 |
| Deoxyribonuclease I: 0.002% | |||||
| 小鼠 | 小鼠, 2-4 mo | Ventricular myocytes | Collagenase Type 2: 0.05% | MEM | Zhou YY, Wang SQ, Zhu WZ, Chruscinski A, Kobilka BK, Ziman B, Wang S, Lakatta EG, Cheng H, Xiao RP: Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology, Am J Physiol Heart Circ Physiol 279, H429-36, 2000 |
| Collagenase Type 4: 0.05% | |||||
| Protease XIV: 0.002% | |||||
| 小鼠 | 小鼠 | Cardiomyocytes | Collagenase Type 2: 150 u/ml | Joklik's MEM | Christensen G, Minamisawa S, Gruber PJ, Wang Y, Chien KR: High-efficiency, long-term cardiac expression of foreign genes in living mouse embryos and neonates, Circulation 101, 178-84, 2000 |
| 小鼠 | 小鼠, 1-3 day | Cardiomyocytes | Trypsin: 0.25% | FBS-MEM | Wang, G., and Kang, Y.: Inhibition of Doxorubicin Toxicity in Cultured Neonatal Mouse Cardiomyocytes with Elevated Metallothionein Levels, J Pharmacol Exp Ther 288(3), 938, 1999 |
| 小鼠 | 小鼠, 1 day | Cardiomyocytes | NCIS kit: with modifications see reference | L-15 | Lader, A., Kwiatkowski, D., and Cantiello, H.: Role of Gelsolin in the Actin Filament Regulation of Cardiac L-type Calcium Channels, Am J Physiol 277, C1277, 1999 |
| 小鼠 | 小鼠, 3-7 month | Fibroblasts, mesangial, smooth muscle | Trypsin: 0.25% | DMEM | Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH: Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation, Mol Biol Cell 10, 1569-79, 1999 |
| Collagenase: see reference | |||||
| Soybean Trypsin Inhibitor: .05% | |||||
| 小鼠 | 小鼠 (BALB/c), adult | Myocytes | Collagenase Type 2: 0.04% | Tyrode's solution | Felzen, B., Shilkrut, M., Less, H., Sarapov, I., Maor, G., Coleman, R., Robinson, R., et al.: Fas (CD95/Apo-1)-Mediated Damage to Ventricular Myocytes Induced by Cytotoxic T Lymphocytes from Perforin-Deficient Mice,Circ Res 82, 438, 1998 |
| 小鼠 | 小鼠, 1 day | Cardiomyocytes | NCIS kit: with modifications see reference | L-15 | Lader, A., Xiao, Y., Ishikawa, Y., Cui, Y., Vatner, D., Vatner, S., Homcy, C., Cantiello, H.: Cardiac Gsalpha Overexpression Enhances L-type Calcium Channels Through an Adenylyl Cyclase Independent Pathway, Proc Natl Acad Sci U S A 95, 9669, 1998 |
| 小鼠 | 小鼠, P0-P1 | Cardiomyocytes | Collagenase: 0.045% | DMEM, Medium 199 | Arber, S., Hunter, J., Ross, J., Hongo, M., Sansig, G., Borg, J., Perriard, J., Chien, K., and Caroni, P.: MLP-Deficient Mice Exhibit a Disruption of Cardiac Cytoarchitectural Organization, Dilated Cadiomyopathy, and Heart Failure,Cell 88, 393, 1997 |
| 小鼠 | 小鼠, 6-9 or 21-41 day | Ventricular and atrial myocytes | Collagenase Type 2: 95 u/ml | DMEM/Tyrodes | Valenzuela, D., Han, X., Mende, U., Fankhauser, C., Mashimo, H., Huang, P., Pfeffer, J., Neer, E., and Fishman, M.: G alpha(o) is Necessary for Muscarinic Regulation of Ca2+ Channels in Mouse Heart, Proc Natl Acad Sci U S A 94(5), 1727, 1997 |
| Hyaluronidase: 172.5 u/ml | |||||
| Trypsin: 0.002% | |||||
| Deoxyribonuclease I: 60u/ml | |||||
| 小鼠 | 小鼠 | Cardiomyocyte | Collagenase: 0.17 % | PBS | Soonpaa, M., Kim, K., Pajak, aL., Franklin, M., and Field, L.:Cardiomyocyte DNA Synthesis and Binucleation During Murine Development,Am J Physiol 271, H2183, 1996 |
| 小鼠 | 小鼠 embryos | Myocytes | Collagenase Type 1: 0.1% | DMEM | Wobus, A., Kleppisch, T., Maltsev, V., and Hescheler, J.: Cardiomyocyte-Like Cells Differentiated In Vitro From Embryonic Carcinoma Cells P19 are Characterized by Functional Expression of Adrenoceptors and Ca2+ Channels,In Vitro Cell Dev Biol 30A, 425, 1994 |
| 小鼠 | 小鼠 | Myocytes | Trypsin NF 1:250: 0.25% | Rinaldini's buffer solution, CF | Gross, W., Schopf-Ebner, E., and Bucher, O.: Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells, Exp Cell Res 53, 1, 1968 |
| (also rat, neonate and chick, 8 day embryo) | |||||
| 綿羊 | 綿羊 | Atrial myocytes | Collagenase Type 2: 0.055% | Tyrode's solution | Voigt, N., Pearman, C., Dobrev, D. and Dibb, K.: Methods for Isolating Atrial Cells from Large Mammals and Humans., J Mol Cell Cardiol 86, 187-98, 2015 |
| Protease XIV: 0.006% | |||||
| 綿羊 | 綿羊 | Cardiomyocytes | Collagenase Type 2: 0.055% | See reference | Dibb, K., Clarke, J., Horn, M., Richards, M., Graham, H., Eisner, D. and Trafford, A.: Characterization of an Extensive Transverse Tubular Network in Sheep Atrial Myocytes and its Depletion in Heart Failure., Circ Heart Fail 2, 482-9, 2009 |
| Protease Type XIV: 0.006% | |||||
| 綿羊 | 綿羊 | Cardiomyocytes | Collagenase Type 2: 100-200 u/ml | See reference | Dhamoon, A, Pandit, S., Sarmast, F., Parisian, K., Guha, P., Li, Y., Bagwe, S., Taffet, S. and Anumonwo, J.: Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current.,Circ Res 94, 1332-9, 2004 |
| 豬 | 豬 | Aortic smooth muscle and intersitial cells | Collagenase: 600 u/ml | PBS | Butcher, J. and Nerem, R.: Porcine Aortic Valve Interstitial Cells in Three-Dimensional Culture: Comparison of Phenotype with Aortic Smooth Muscle Cells., J Heart Valve Dis 13, 478, 2004 |
| 豬 | 豬 | Coronary vascular smooth muscle (PCVSMCs) | Trypsin: 0.037% | HEPES | Christ, M., Gunther, A., Heck, M., Schmidt, B., Falkenstein, E., and Wehling, M.: Aldosterone, Not Estradiol, Is the Physiological Agonist for Rapid Increases in cAMP in Vascular Smooth Muscle Cells, Circulation 99, 1485, 1999 |
| 豬 | Miniature swine, adult, female, 25-40 kg, familiarized with treadmill exercise | Coronary smooth muscle | Trypsin: 0.1% | Krebs bicarbonate solution | Bowles, D., Hu, Q., Laughlin, M., and Sturek, M.: Exercise Training Increases L-type Calcium Current Density in Coronary Smooth Muscle, Am J Physiol 275 (44), H2159, 1998 |
| 兔 | 兔, New Zealand, 2.3-2.9 kg | Cardiomyocytes | Collagenase Type 1: 0.05% | Krebs-Henseleit | Farkas, A., Acsai, K., Nagy, N., Toth, A., Fulop, F., Seprenyi, G., Birinyi, P., Nanasi, P., Forster, T., Csanady, M., Papp, J., Varrro, A. and Farkas, A.:Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart., Br J Pharmacol 154, 93, 2008 |
| Hyaluronidase: see reference | |||||
| Protease: see reference | |||||
| 兔 | 兔 | Smooth muscle cells | Collagenase Type 2: 300 u/ml | F10 Ham | Verheye, S., Martinet, W., Kockx, M., Knaapen, M., Salu, K., Timmermans, J., Ellis, J., Kilpatrick, D., DeMayer, G.: Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy, J Am Coll Cardiol49, 706, 2007 |
| Elastase: 5 u/ml | |||||
| 兔 | 兔, New Zealand, 2 kg | Cardiomyocytes and fibroblasts | Collagenase: 0.06% | Medium 199 | Driesen Ronald B, Dispersyn Gerrit D, Verheyen Fons K, van den Eijnde Stefan M, Hofstra Leo, Thone Fred, Dijkstra Petra, Debie Wiel, Borgers Marcel, Ramaekers Frans C S: Partial cell fusion: a newly recognized type of communication between dedifferentiating cardiomyocytes and fibroblasts,Cardiovasc Res 68, 37-46, 2005 |
| 兔 | 兔, New Zealand White, 2-3 kg | Myocytes | Collagenase Type 2: 0.1% | HEPES | Spitzer, K., Ershler, P., Skolnick, R., and Vaughan-Jones, R.: Generation of Intracellular pH Gradients in Single Cardiac Myocytes with a Microperfusion System, Am J Physiol 278, H1371, 2000 |
| 兔 | 兔, New Zealand, white, either sex | Myocytes | Collagenase Type 2: 0.05% | EGTA-KB | Sedarat, F., Xu, L., Moore, E., and Tibbits, G.: Colocalization of Dihydropyridine and Ryanodine Receptors in Neonate Rabbit Heart Using Confocal Microscopy, Am J Physiol Heart Circ Physiol 279, H202, 2000 |
| 兔 | 兔, adult 1.5-2.5 kg | Tricuspid valve cells | Collagenase: 0.8 mg/ml | HEPES Tyrode solution | Anumonwo JM, Delmar M, and Jalife J.: Electrophysiology of single heart cells from the rabbit tricuspid valve, J Physiol 425, 145, 1990 |
| 兔 | 兔, New Zealand, white, male, 2-3 kg | Cardiomyocytes | Hyaluronidase: 0.5% | Eagle's MEM | Buxton, I., and Brunton, L.: Compartments of Cyclic AMP and Protein Kinase in Mammalian Cardiomyocytes, J Biol Chem 258 (17), 10233, 1983 |
| 兔 | 兔, adult, 2 kg | Myocytes | Hyaluronidase: 0.007% | Kreb's Ringer | Dani, A., Cittadini, A., Flamini, G., Festuccia, G., and Terranova, T.:Preparation and Some Properties of Isolated Beating Myocytes from Adult Rabbit Heart, J Mol Cell Cardiol 9, 777, 1977 |
| 大鼠 | 大鼠, 1-2 day | Cardiomyocytes | NCIS kit: per instructions | MEM | Xie, L., Pi, X., Townley-Tilson, WH., Li, N., Wehrens, X., Entman, M., Taffet, G., Mishra, A., Peng, J., Schisler, J., Meissner, G. and Patterson, C.: PHD2/3-dependent Hydroxylation Tunes Cardiac Response to 尾-adrenergic Stress via Phospholamban., J Clin Invest 125, 2759-71, 2015 |
| 大鼠 | 大鼠, Wistar, 250-350g | Ventricular myocytes | Collagenase Type 2: 251 u/ml | Tyrode solution | Calderon-Sachez, E., Domi璶guez-Rodriguez, A., Lopez-Haldon, J., Jimenez-Navarro, M., Gomez, A., Smani, T. and Ordonez, A.:Cardioprotective Effect of Ranolazine in the Process of Ischemia-reperfusion in Adult Rat Cardiomyocytes., Rev Esp Cardiol (Engl Ed) , , 2015 |
| 大鼠 | 大鼠, SD, 8-10 week | Ventricular myocytes | Collagenase: 0.08% | Tyrode's Solution | Lee, J., Ha, J. and Leem, C.: A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca(2+) Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat., Korean J Physiol Pharmacol 19, 373-82, 2015 |
| Pronase: 0.004% | |||||
| 大鼠 | 大鼠, SD, 1 day | Cardiomyocytes | Trypsin: 0.025% | HBSS | Clark, A. and Naya, F.: MicroRNAs in the Myocyte Enhancer Factor 2 (MEF2)-regulated Gtl2-Dio3 Noncoding RNA Locus Promote Cardiomyocyte Proliferation by Targeting the Transcriptional Coactivator Cited2., J Biol Chem 290, 23162-72, 2015 |
| Collagenase Type 2: 1.0% | |||||
| 大鼠 | 大鼠, SD, 2-3 day | Cardiomyocytes | Collagenase Type 2: 0.05% | DMEM | Fang, R, Qiao, S., Liu, Y., Meng, Q., Chen, X., Song, B., Hou, X. and Tian, W.: Sustained Co-Delivery of BIO and IGF-1 by a Novel Hybrid Hydrogel System to Stimulate Endogenous Cardiac Repair in Myocardial Infarcted Rat Hearts., Int J Nanomedicine 10, 4691-703, 2015 |
| 大鼠 | 大鼠, SD | Valve Interstitial cells | Collagenase Type 2: 600 u/ml | DMEM | Horne, T., VandeKopple, M., Sauls, K., Koenig, S., Anstine, L., Garg, V., Norris, R. and Lincoln, J.: Dynamic Heterogeneity of the Heart Valve Interstitial Cell Population in Mitral Valve Health and Disease, J Cardiovasc Dev Dis 2, 214, 2015 |
| 大鼠 | Wistar, 200 g | Ventricular myocytes | Collagenase Type 2: 95 u/ml | HEPES | Miller, L., Greensmith, D., Sankaranarayanan, R., O'Neill, S. and Eisner D.:The Effect of 2,5-di-(tert-butyl)-1,4-benzohydroquinone (TBQ) on Intracellular Ca2+ Handling in Rat Ventricular Myocytes., Cell Calcium 58, 208-14, 2015 |
| Protease: 0.1 u/ml | |||||
| 大鼠 | 大鼠, SD, 3-4 month | Cardiomyocytes | Collagenase Type 2: 0.1% | DMEM | Liu, L., Wang, Y., Cao, Z., Wang, M., Liu, X., Gao, T., Hu, Q., Yuan, W. and Lin, L.: Up-regulated TLR4 in Cardiomyocytes Exacerbates Heart Failure after Long-Term Myocardial Infarction., J Cell Mol Med 19, 2728, 2015 |
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | NCIS kit: per instructions | HBSS | Song, D., Lee, K., Ryu, J., Jeon, H. and Kim, D.: The Molecular Interaction of Heart LIM Protein (HLP) with RyR2 and Caveolin-3 is Essential for Ca(2+)-Induced Ca(2+) Release in the Heart., Biochem Biophys Res Commun 463, 975-81, 2015 |
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | Trypsin: 0.06% | HBSS | Zhang, B., Montgomery, M., Davenport-Huyer, L., Korolj, A. and Radisic, M.: Platform Technology for Scalable Assembly of Instantaneously Functional Mosaic Tissues., Sci. Adv. 1, e1500423, 2015 |
| Collagenase Type 2: 220 u/ml | |||||
| 大鼠 | 大鼠 | Cardiomyocytes | Collagenase Type 2: 150 u/ml | MEM | Hennessey, J., Wei, E. and Pitt, G.: Fibroblast Growth Factor Homologous Factors Modulate Cardiac Calcium Channels., Circ Res 113, 381-8, 2013 |
| 大鼠 | 大鼠, Wistar, 175-200g | Cardiomyocytes | Collagenase Type 2: 150 u/ml | see reference | Eckerle, L., Felix, S. and Herda, L.: Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes., J Vis Exp 73, e4237, 2013 |
| 大鼠 | 大鼠, SD, 1-2 day | Cardiomyocytes | Trypsin: 0.06% | DMEM | Zhang, B., Green, J., Murthy, S. and Radisic, M.: Label-Free Enrichment of Functional Cardiomyocytes using Microfluidic Deterministic Lateral Flow Displacement., PLoS ONE 7, e37619, 2012 |
| Collagenase Type 2: 220 u/ml | |||||
| 大鼠 | 大鼠, adult | Ventricular myocytes | Collagenase Type 2: 0.2% | Medium 199 | Karakikes, I., Hadri, L., Rapti, K., Ladage, D., Ishikawa, K., Tilemann, L., Yi, G., Morel, C., Gwathmey, J., Zsebo, K., Weber, T., Kawase, Y. and Hajjar, R.: Concomitant Intravenous Nitroglycerin with Intracoronary Delivery of AAV1.SERCA2a Enhances Gene Transfer in Porcine Hearts., Mol Therapy 20, 565, 2012 |
| 大鼠 | 大鼠, 3 day | Cardiomyocytes | Collagenase Type 2: 300 u/ml | PBS | Ye, K., Sullivan, K. and Black, L.: Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering., J Vis Exp 55, 3251, 2011 |
| 大鼠 | 大鼠, SD, male | Cardiomyocytes | Collagenase Type 2: 0.056% | Krebs | Mellor, K., Bell, J., Wendt, I., Davidoff, A., Ritchie, R. and Delbridge, L.:Fructose Modulates Cardiomyocyte Excitation-Contraction Coupling and Ca Handling In Vitro., PLoS ONE 6, e25204, 2011 |
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | NCIS kit: per instructions | L-15 | Dittami, G., Rajguru, S., Lasher, R., Hitchcock, R. and Rabbitt, R.:Intracellular Calcium Transients Evoked by Pulsed Infrared Radiation in Neonatal Cardiomyocytes., J Physiol 589, 1295-306, 2011 |
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | Trypsin: 0.07% | DMEM/M199 | Guan, X., Delo, D., Atala, A. and Soker, S.: In Vitro Cardiomyogenic Potential of Human Amniotic Fluid Stem Cells., J Tissue Eng Regen Med 5, 220, 2011 |
| Collagenase Type 2: 0.1% | |||||
| 大鼠 | 大鼠, adult | Cardiomyocytes | Collagenase Type 2: 0.025% | see reference | Louch, W., Sheehan, K. and Wolska, B.: Methods in Cardiomyocyte Isolation, Culture, and Gene Transfer., J Mol Cell Cardiol 51, 288-98, 2011 |
| 大鼠 | 大鼠, Wistar, male | Cardiomyocytes | NCIS kit: per instructions | L-15 | Brinckmann, M., Kaschina, E., Altarche-Xifro, W., Curato, C., Timm, M., Grzesiak, A., Dong, J., Kappert, K., Kintscher, U., Unger, T. and Li, J.:Estrogen Receptor Alpha Supports Cardiomyocytes Indirectly Through Post-Infarct Cardiac c-kit+ Cells., J Mol Cell Cardiol 47, 66, 2009 |
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | NCIS kit: per instructions | L-15 | Smith, M., Huang, Y. and Deshmukh, M.: Skeletal Muscle Differentiation Evokes Endogenous XIAP to Restrict the Apoptotic Pathway., PLoS ONE 4, e5097, 2009 |
| 大鼠 | 大鼠, adult | Myocytes | Collagenase Type 2: 0.05% | Media 199 | Xu, X. and Colecraft, H.: Primary Culture of Adult Rat Heart Myocytes., J Vis Exp 28, 1308, 2009 |
| Protease XIV: 0.02% | |||||
| 大鼠 | 大鼠, SD, 200-250 g | Cardiomyocytes | Collagenase Type 1: 0.05% | Krebs-Henseleit | Farkas, A., Acsai, K., Nagy, N., Toth, A., Fulop, F., Seprenyi, G., Birinyi, P., Nanasi, P., Forster, T., Csanady, M., Papp, J., Varrro, A. and Farkas, A.:Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart., Br J Pharmacol 154, 93, 2008 |
| Hyaluronidase: see reference | |||||
| Protease: see reference | |||||
| 大鼠 | 大鼠, SD, 1-2 day | Cardiomyocytes | NCIS kit: per instructions | L-15 | Kim, M., Oh, J., Sakata, S., Liang, I., Park, W., Hajjar, R. and Lebeche, D.:Role of Resistin in Cardiac Contractility and Hypertrophy., J Mol Cell Cardiol 45, 270, 2008 |
| 大鼠 | 大鼠, SD, 2 day | Cardiomyocytes | Collagenase Type 2: 0.1% | DMEM/F-12 | Jang, J., Ku, S., Kim, J., Choi, K., Kim, Y., Kim, H., Oh, S., Lee, E., Cho, H., Song, Y., Lee, S., Lee, S., Suh, C., Kim, S., Moon, S. and Choi, Y.: Notch Inhibition Promotes Human Embryonic Stem Cell-Derived Cardiac Mesoderm Differentiation., Stem Cells 26, 2782, 2008 |
| 大鼠 | 大鼠, SD, male, 225-250g | Cardiomyocytes | Collagenase Type 2: 0.1% | J-MEM | Kubli, D., Quinsay, M., Huang, C., Lee, Y., Gustafsson, A.: Bnip3 Functions as a Mitochondrial Sensor of Oxidative Stress During Myocardial Ischemia and Reperfusion., Am J Physiol Heart Circ Physiol 295, H2025, 2008 |
| 大鼠 | 大鼠, 2-3 day | Cardiomyocytes | Collagenase Type 2: 0.05% | DMEM | LaFramboise, W., Scalise, D., Stoodley, P., Graner, S., Guthrie, R., Magovern, J and Becich, M.: Cardiac Fibroblasts Influence Cardiomyocyte Phenotype in Vitro., Am J Physiol Cell Physiol 292, C1799-808, 2007 |
| Pancreatin: 0.1% | |||||
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | NCIS kit: per instructions | L-15 | Robinet, A., Millart, H., Oszust, F., Hornebeck, W. and Bellon, G.: Binding of Elastin Peptides to S-Gal Protects the Heart Against Ischemia/Reperfusion Injury by Triggering the RISK Pathway., FASEB J 21, 1968, 2007 |
| 大鼠 | 大鼠, SD, 300-350 g | Cardiomyocytes | Collagenase Type 2: 0.05% | DMEM | Niederbichler Andreas D, Hoesel Laszlo M, Westfall Margaret V, Gao Hongwei, Ipaktchi Kyros R, Sun Lei, Zetoune Firas S, Su Grace L, Arbabi Saman, Sarma J Vidya, Wang StewartC, Hemmila MarkR, Ward PeterA:An essential role for complement C in the pathogenesis of septic cardiac dysfunction, J Exp Med 203, 53-61, 2006 |
| Hyaluronidase: 0.02% | |||||
| 大鼠 | 大鼠, SD, male | Ventricular myocytes | Collagenase: 0.13% | DMEM | Stagg Mark A, Coppen Steven R, Suzuki Ken, Varela-Carver Anabel, Lee Joon, Brand Nigel J, Fukushima Satsuki, Yacoub Magdi H, Terracciano Cesare M: Evaluation of frequency, type, and function of gap junctions between skeletal myoblasts overexpressing connexin43 and cardiomyocytes: relevance to cell transplantation, FASEB J 20, 744-6, 2006 |
| Hyaluronidase: 0.06% | |||||
| 大鼠 | 大鼠, SD, 200-250 g | Ventricular myocytes and mesenteric arterial SMC | Collagenase Type 2: 0.06% | Krebs-Ringer | Lu Tong, Ye Dan, Wang Xiaoli, Seubert John M, Graves Joan P, Bradbury J Alyce, Zeldin Darryl C, Lee Hon-Chi: Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms, J Physiol 575, 627-44, 2006 |
| Papain: 0.175% | |||||
| 大鼠 | 大鼠, Wistar, neonatal | Cardiomyocytes | NCIS kit: per instructions | L-15 | Butler, T., Au, C., Yang, B., Egan, J., Tan, Y., Hardeman, E., North, K., Verkman, A. and Winlaw, D.: Cardiac Aquaporin Expression in Humans, Rats, and Mice., Am J Physiol Heart Circ Physiol 291, H705, 2006 |
| 大鼠 | 大鼠, 1-2 day | Cardiomyocytes | NCIS kit: per instructions | DMEM/F-12 | Pedram, A., Razandi, M., Aitkenhead, M., and Levin, E: Estrogen Inhibits Cardiomyocyte Hypertrophy in vitro. Antagonism of Calcineurin-related Hypertrophy through Induction of MCIP1, J Biol Chem 280, 26339, 2005 |
| 大鼠 | 大鼠, SD, male, 250-300 g | Cardiomyocytes | Collagenase Type 2: 250 u/ml | Cardioplegic solution | Head, B., Pa, H., Roth, D., Lai, N., Niesman, I., Farquhar, M. and Insel, P.: G-Protein-Coupled Receptor Signaling Components Localize in Both Sarcolemmal and Intracellular Caveolin-3-Associated Microdomains in Adult Cardiac Myocytes., J Biol Chem 280, 31036-44, 2005 |
| 大鼠 | 大鼠, Wistar, 1-3 day | Cardiomyocytes | NCIS kit: per instructions | L-15 | Castillo, A., Ruzmetov, N., Harvey, K., Stillwell, W., Zaloga, G. and Siddiqui, R.: Docosahexaenoic Acid Inhibits Protein Kinase C Translocation/Activation and Cardiac Hypertrophy in Rat Cardiomyocytes., J Mol Genet Med 1, 18, 2005 |
| 大鼠 | 大鼠, SD, 2-4 day | Cardiomyocytes, fibroblasts | Trypsin: 0.1% | Medium 199 | Entcheva, E., Bien, H, Yin L., Chung, C., Farrell, M., and Kostov, Y.:Functional Cardiac Cell Constructs on Cellulose-based Scaffolding,Biomaterials 25(26), 5753, 2004 |
| Collagenase: 0.1% | |||||
| 大鼠 | 大鼠, SD, 1-2 day | Cardiomyocytes | NCIS kit: per instructions | L-15 | Muller-Bore, B., Cascio, W., Anderson, P., Snowwaert, J., Frye, J., Desai, N., Esch, G., Brackham, J., Bagnell, C., Coleman, W., Grisham, J., and Malouf, N.: Adult-derived Liver Stem Cells Acquire a Cardiomyocyte Structural and Functional Phenotype ex vivo, Am J Pathol 165, 135, 2004 |
| 大鼠 | 大鼠,1-2 day | Cardiomyocytes | NCIS kit: per instructions | DMEM | Natarajan AR, Rong Q, Katchman AN, and Ebert SN.: Intrinsic cardiac catecholamines help maintain beating activity in neonatal rat cardiomyocyte cultures, Pediatr Res 56, 411, 2004 |
| 大鼠 | 大鼠, 1 day | Cardiomyocytes | NCIS kit: per instructions | F-10 | Chen, Z., Ge, Y., and Kang J.: Down-regulation of the M6P/IGF-II Receptor Increases Cell Proliferation and Reduces Apoptosis in Neonatal Rat Cardiac Myocytes, BMC Cell Biol 5, 15, 2004 |
| 大鼠 | 大鼠, neonatal | Cardiac myocytes | Collagenase Type 2: 0.5% | HBSS | Chen Hsiao-Huei, Mullett Steven J, Stewart Alexandre F R: Vgl-4, a novel member of the vestigial-like family of transcription cofactors, regulates alpha1-adrenergic activation of gene expression in cardiac myocytes, J Biol Chem 279, 30800-6, 2004 |
| 大鼠 | 大鼠, male, 250-300 g | Cardiomyocytes | Collagenase Type 1: 0.03% | HBSS | Hunton DaciaL, Zou LuYun, Pang Yi, Marchase Richard B: Adult rat cardiomyocytes exhibit capacitative calcium entry, Am J Physiol Heart Circ Physiol 286, H1124-32, 2004 |
| Protease: 0.01% | |||||
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | Collagenase Type 2: 0.1% | DMEM | Tamamori-Adachi, M., Hayashida, K., Nobori, K., Omizu, C., Yamada, K., Sakamoto, N., Kamura, T., Fukuda, K., Ogawa, S., Nakayama, K. and Kitajima, S.: Down-Regulation of p27 Promotes Cell Proliferation of Rat Neonatal Cardiomyocytes Induced by Nuclear Expression of Cyclin D1 and CDK4, J Biol Chem 2279, 50429, 2004 |
| 大鼠 | 大鼠, fetal or 3-day | Cardiomyocytes | Collagenase Type 2: 0.1% | HEPES | Tamamori-Adachi Mimi, Ito Hiroshi, Sumrejkanchanakij Piyamas, Adachi Susumu, Hiroe Michiaki, Shimizu Masato, Kawauchi Junya, Sunamori Makoto, Marumo Fumiaki, Kitajima Shigetaka, Ikeda Masa-Aki: Critical role of cyclin D1 nuclear import in cardiomyocyte proliferation, Circ Res 92, e12-9, 2003 |
| 大鼠 | 大鼠, adult, 220-280g | Cardiomyocytes | Collagenase Type 2: 178 u/ml | Krebs-Henseleit, CF | Gordon Jennifer M, Dusting Gregory J, Woodman Owen L, Ritchie Rebecca H: Cardioprotective action of CRF peptide urocortin against simulated ischemia in adult rat cardiomyocytes, Am J Physiol Heart Circ Physiol 284, H330-6, 2003 |
| Hyaluronidase: 0.01% | |||||
| 大鼠 | 大鼠, 50 g | Ventricular myocytes | Collagenase: 223 u/ml | Medium 199 | Aberle II NS, and Ren J.: Experimental Assessment of the Role of Acetaldehyde in Alcoholic Cardiomyopathy, Biol Proced Online 5, 1, 2003 |
| Hyaluronidase: 0.01% | |||||
| Trypsin: 0.002% | |||||
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | NCIS kit: per instructions | L-15 | Li TS, Ito H, Kajiwara K, and Hamano K.: Long-term survival of xenografted neonatal cardiomyocytes by adenovirus-mediated CTLA4-Ig expression and CD40 blockade, Circulation 108, 1760, 2003 |
| 大鼠 | 大鼠, SD, 200-300 g | Cardiomyocytes | Collagenase Type 2: 0.05-0.1% | Joklik's MEM | Swift Luther, McHowat Jane, Sarvazyan Narine: Inhibition of membrane-associated calcium-independent phospholipase A2 as a potential culprit of anthracycline cardiotoxicity, Cancer Res 63, 5992-8, 2003 |
| 大鼠 | 大鼠, SD, 175-200g | Cardiomyocytes | Collagenase Type 2: 0.05% | Krebs-Ringer | Tardif Annie, Julien Nathalie, Chiasson Jean-Louis, Coderre Lise:Stimulation of glucose uptake by chronic vanadate pretreatment in cardiomyocytes requires PI 3-kinase and p38 MAPK activation, Am J Physiol/Endo 284, E1055-64, 2003 |
| Deoxyribonuclease I: 0.02% | |||||
| 大鼠 | 大鼠, SD, pregnant female | Peritoneal mast | Hyaluronidase: 100 u/ml | DMEM (see reference) | DeAlmeida, A., Mustin, D., Forman, M., Brower, G., Janicki, J., and Carver, W.: Effects of mast cells on the behavior of isolated heart fibroblasts: modulation of collagen remodeling and gene expression, J Cell Physiol 191, 51, 2002 |
| 大鼠 | 大鼠, neonatal, adult, 17 day | Ventricular cardiomyocytes | Collagenase Type 2: 0.7-1% | DMEM | Lam ML, Bartoli M, and Claycomb WC.: The 21-day postnatal rat ventricular cardiac muscle cell in culture as an experimental model to study adult cardiomyocyte gene expression, Mol Cell Biochem 229, 51, 2002 |
| 大鼠 | 大鼠, 1 day | Cardiomyocytes | NCIS kit: per instructions | L-15 | Yang Y, Liao H, Ke Q, Cai J, Xiao YF, and Morgan JP: Enhancement of nitric oxide production by methylecgonidine in cultured neonatal rat cardiomyocytes, Br J Pharmacol 135, 188, 2002 |
| 大鼠 | 大鼠, female, 250-300 g | Ventricular myocytes | Collagenase Type 2: 0.1% | Ringer solution | Liu Qinghang, Hofmann Polly A: Antiadrenergic effects of adenosine A(1) receptor-mediated protein phosphatase 2a activation in the heart, Am J Physiol Heart Circ Physiol 283, H1314-21, 2002 |
| 大鼠 | 大鼠, Wistar, 1 day | Cardiomyocytes | Collagenase Type 2: 80u/ml | M199 | Shimizu Tatsuya, Yamato Masayuki, Isoi Yuki, Akutsu Takumitsu, Setomaru Takeshi, Abe Kazuhiko, Kikuchi Akihiko, Umezu Mitsuo, Okano Teruo: Fabrication of pulsatile cardiac tissue grafts using a novel 3-dimensional cell sheet manipulation technique and temperature-responsive cell culture surfaces, Circ Res 90, e40, 2002 |
| 大鼠 | 大鼠, male, 6-12 months old | Cardiac myocytes | Protease: 0.55 u/ml | Tyrode's solution | Kitta, K., Day, R., Ikeda, T., and Suzuki, Y.: Hepatocyte Growth Factor Protects Cardiac Myocytes Against Oxidative Stress-Induced Apoptosis, Free Radic Biol Med 31 (7), 902, 2001 |
| 大鼠 | 大鼠, Harlan SD, pup | Cardiomyocytes | Trypsin: | DMEM/Ham's F-12 | Schwartzbauer, G., and Robbins, J.: The Tumor Suppressor Gene PTEN Can Regulate Cardiac Hypertrophy and Survival, J Biol Chem 276, 35786, 2001 |
| 大鼠 | 大鼠, SD, newborn | Cardiac myocytes | NCIS kit: per instructions | L-15 | Rahman A, Alam M, Rao S, Cai L, Clark LT, Shafiq S, and Siddiqui MA:Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitro, Biol Res 34(3-4), 195, 2001 |
| 大鼠 | 大鼠, adult | Ventricular myocytes | Collagenase: 223 u/ml | Tyrode solution | Ren J, and Wold LE.: Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging, Biol Proced Online 3, 43, 2001 |
| Hyaluronidase: 0.01% | |||||
| Trypsin: 0.002% | |||||
| 大鼠 | 大鼠, SD, male | Cardiomyocytes | Collagenase Type 2: 140 u/ml | Krebs-Henseleit | Dai L, Brookes PS, Darley-Usmar VM, and Anderson PG.: Bioenergetics in cardiac hypertrophy: mitochondrial respiration as a pathological target of NO, Am J Physiol/Heart 281, H2261-H2269, 2001 |
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | NCIS kit: per instructions | DMEM/M199 | Guo K, Searfoss G, Krolikowski D, Pagnoni M, Franks C, Clark K, Yu KT, Jaye M, Ivashchenko Y: Hypoxia induces the expression of the pro-apoptotic gene BNIP3, Cell Death Differ 8, 367-76, 2001 |
| 大鼠 | 大鼠, Wistar, female | Ventricular myocytes | Collagenase Type 2: 0.1% | Ringer solution | Pyle, W, Lester, J, and Hofmann, P.: Effects of Kappa-opioid receptor activation of myocardium, Am J Physiol Heart Circ Physiol 281, H669, 2001 |
| 大鼠 | 大鼠, SD, 1-2 day | Cardiomyocytes | Trypsin: 0.01% | HBSS | Arutunyan A, Webster D, Swift L, Sarvazyan N: Localized injury in cardiomyocyte network: a new experimental model of ischemia-reperfusion arrhythmias, Am J Physiol Heart Circ Physiol 280, H1905-15, 2001 |
| Collagenase Type 2: 0.08% | |||||
| 大鼠 | 大鼠, newborn | Atrial | Trypsin: 0.06% | DMEM | Kim, D., and Pleumsamran, A.: Cytoplasmic Unsaturated Free Fatty Acids Inhibit ATP-Dependent Gating of the G Protein-gated K+ Channel, J Gen Physiol 115, 287, 2000 |
| 大鼠 | 大鼠, 1-2 day | Cardiomyocytes | Collagenase Type 1: 84 u/ml | M199 medium | Bueno OF, De Windt LJ, Tymitz KM, Witt SA, Kimball TR, Klevitsky R, Hewett TE, Jones SP, Lefer DJ, Peng CF, Kitsis RN, and Molkentin JD.: The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice, EMBO J 19(23), 6341, 2000 |
| Pancreatin: 0.05% | |||||
| 大鼠 | 大鼠, Winstar, 1-2 day | Ventricular myocytes | Collagenase Type 2: 0.08% | DMEM | Maki T, Horio T, Yoshihara F, Suga S, Takeo S, Matsuo H, and Kangawa K: Effect of neutral endopeptidase inhibitor on endogenous atrial natriuretic peptide as a paracrine factor in cultured cardiac fibroblasts, Br J Pharmacol 131(6), 1204, 2000 |
| 大鼠 | 大鼠, SD, adult female | Heart microvascular cells | CLSPA: 250u/ml | L-15 | Puri S, Bansal DD, Uskokovic MR, and MacGregor RR.: Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)(2)D(3), Am J Physiol/Endo 278(2), E293, 2000 |
| Papain: 5 u/ml | |||||
| Elastase: 0.8 u/ml | |||||
| 大鼠 | 大鼠, SD, male, 250-300g | Cardiomyocytes | Collagenase Type 2: 0.06% | M199 | Sun L, Chang J, Kirchhoff SR, and Knowlton AA.: Activation of HSF and selective increase in heat-shock proteins by acute dexamethasone treatment, Am J Physiol Heart Circ Physiol 278(4), H1091, 2000 |
| 大鼠 | 大鼠, wistar, 2 day | Cardiomyocytes | NCIS kit: per instructions | DMEM-F12 | Cowan DB, Poutias DN, Del Nido PJ, and McGowan FX Jr: CD14-independent activation of cardiomyocyte signal transduction by bacterial endotoxin, Am J Physiol Heart Circ Physiol 279, H619, 2000 |
| 大鼠 | 大鼠, neonatal | Cardiomyocytes | NCIS kit: per instructions | L-15 | Webster DR, and Patrick DL.: Beating rate of isolated neonatal cardiomyocytes is regulated by the stable microtubule subset, Am J Physiol Heart Circ Physiol 278, H1653, 2000 |
| 大鼠 | 大鼠, Lewis, adult male, 300-350-g | Aorta, smooth muscle | Trypsin: 0.025% | Medium 199 with 20% FBS medium | Kim, B., Nikolovski, J., Bonadio, J., Smiley, E., and Mooney, D.: Engineered Smooth Muscle Tissues: Regulating Cell Phenotype with the Scaffold, Exp Cell Res 251, 318, 1999 |
| 大鼠 | 大鼠, 10-15 newborn, 1-2 days old | Atrial | Trypsin: 0.03% | HBSS or EBSS | Nemec, J., Wickman, K., and Clapham, D.: GBy Binding Increases the Open Time of IKACh: Kinetic Evidence for Multiple GBy Binding Sites, Biophys J 76, 246, 1999 |
| 大鼠 | 大鼠, 1-3 day | Neonatal rat cardiomyocytes | Collagenase Type 2: 80u/ml | DMEM/F12 | Adderley SR, and Fitzgerald DJ.: Oxidative damage of cardiomyocytes is limited by extracellular regulated kinases 1/2-mediated induction of cyclooxygenase-2, J Biol Chem 274, 5038, 1999 |
| Pancreatin: 0.06% | |||||
| 大鼠 | 大鼠, 1-2 day | Cardiomyocytes | Pancreatin: 0.06% | DMEM | Reinecke H, Zhang M, Bartosek T, and Murry CE.: Survival, integration, and differentiation of cardiomyocyte grafts: a study in normal and injured rat hearts, Circulation 100(2), 193, 1999 |
| Collagenase Type 2: 95 u/ml | |||||
| 大鼠 | 大鼠, adult and neonatal | Cardioomyocytes | Collagenase Type 4: 0.12%-adult | DMEM/medium 199 | Richards SM, Jaconi ME, Vassort G, and Puceat M.: A spliced variant of AE1 gene encodes a truncated form of Band 3 in heart: the predominant anion exchanger in ventricular myocytes, J Cell Sci 112, 1519, 1999 |
| Collagenase Type 2: 0.05%-neonatal | |||||
| 大鼠 | 大鼠, Wistar, male | Cardiomyocytes | Collagenase Type 2: 228 u/ml | Joklik MEM | Sambandam N, Abrahani MA, St Pierre E, Al-Atar O, Cam MC, and Rodrigues B.: Localization of lipoprotein lipase in the diabetic heart: regulation by acute changes in insulin, Arterioscler Thromb Vasc Biol 19(6), 1526, 1999 |
| 大鼠 | 大鼠, 1-2 day | Cardiomyocytes | NCIS kit: per instructions | RPMI | Matsui, T., Li, Ling, Del Monte, F., Fukui, Y., Franke, T., Hajjar, R., Rosenzweig, A.: Adenoviral gene transfer of activated phosphatidylinositol 3'-kinase and Akt inhibits apoptosis of hypoxic cardiomyocytes in vitro,Circulation 100, 2373-9, 1999 |
| 大鼠 | 大鼠 | Thoracic aorta, smooth muscle | Elastase: 0.02% | (see reference) | Zilberman, A., Dave, V., Miano, J., Olson, E., and Periasamy, M.:Evolutionary Conserved Promoter Region Containing CArg-Like Elements is Crucial for Smooth Muscle Myosin Heavy Chain Gene Expression, Circ Res 82, 566, 1998 |
| 大鼠 | 大鼠, SD, 1 day | Cardiomyocytes | NCIS kit: per instructions | DMEM/F-12 | Wagner DR, Combes A, McTiernan C, Sanders VJ, Lemster B, Feldman AM: Adenosine inhibits lipopolysaccharide-induced cardiac expression of tumor necrosis factor-alpha, Circ Res 82, 47-56, 1998 |
| 大鼠 | 大鼠, SD, 300-350 g | Cardiomyocytes | Collagenase Type 2: 0.04% | see reference | Yu L, Netticadan T, Xu YJ, Panagia V, Dhalla NS: Mechanisms of lysophosphatidylcholine-induced increase in intracellular calcium in rat cardiomyocytes, J Pharmacol Exp Ther 286, 1-8, 1998 |
| 大鼠 | 大鼠, SD, female, 200g | Ventricular cardiac myocytes | Collagenase Type 2: 0.5% | Krebs-Henseleit buffer | Westfall M V, Rust E M, Metzger J M: Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function, Proc Natl Acad Sci U S A 94, 5444-9, 1997 |
| Hyaluronidase: 0.2% | |||||
| 大鼠 | 大鼠, Wistar, 1 day | Cardiomyoctes | Collagenase Type 2: 80 u/ml | HBSS | Kinugawa, K., Kohmoto, O., Yao, A., Serizawa, T., and Takahashki, T.:Cardiac inducible nitric oxide synthase negatively modulates myocardial function in cultured rat myocytes, Am J Physiol 272, H35-47, 1997 |
| 大鼠 | 大鼠, neonatal, 5-day | Cardiomyocytes | Trypsin: 0.05% | DMEM | Vanwinkle, W., Snuggs, M., and Buja, L.: Cardiogel: A Biosynthetic Extracellular Matrix for Cardiomyocyte Culture, In Vitro Cell Dev Biol Anim 32, 478, 1996 |
| 大鼠 | 大鼠, SD, 250-300 g | Cardiomyocytes | Collagenase Type 2: 0.05% | Joklik's | Sharma VK, Colecraft HM, Wang DX, Levey AI, Grigorenko EV, Yeh HH, and Sheu SS: Molecular and functional identification of m1 muscarinic acetylcholine receptors in rat ventricular myocytes, Circ Res 79(1), 86, 1996 |
| 大鼠 | 大鼠, SD, fetal and neonatal | Cardiomyocytes | Collagenase: 0.1% | PBS | Li RK, Mickle DA, Weisel RD, Zhang J, Mohabeer MK: In vivo survival and function of transplanted rat cardiomyocytes, Circ Res 78, 283-8, 1996 |
| Trypsin: 0.2% | |||||
| 大鼠 | SD, SHR or WKY | Cardiomyocytes | NCIS kit: per instructions | HBSS | MacGregor, R., Klein, R., and Bansal, D.: Secretion of Plasminogen Activator Activity from Neonatal Rat Heart Cells is Regulated by Hormones and Growth Factors, Ann N Y Acad Sci 752, 331, 1995 |
| 大鼠 | 大鼠, SD, male, 200-250 g | Myocytes | Collagenase: 0.07% | Joklik's MEM | Laughlin, M., Schaeffer, M., and Sturek, M.: Effect of Exercise Training on Intracellular Free Ca2+ Transients in Ventricular Myocytes of Rats, Am J Physiol 73, 1141, 1992 |
| Ventricles | |||||
| 大鼠 | 大鼠, SD, female, 60-90 days | Myocardial | Collagenase Type 2: 0.1% | Joklik's MEM, Kreb's-Henseleit buffer, CF | Welder, A.A., Grant, R., Bradlaw, J., and Acosta, D.: A Primary Culture System of Adult Rat Heart Cells for the Study of Toxicologic Agents, In Vitro Cell Dev Biol 27, 921, 1991 |
| 大鼠 | 大鼠, SD, adult, female, 180-220 g | Cardiomyocytes | Collagenase: 0.11% | (see reference) | Fischer, Y., Rose, H., and Kammermeier. H.: Highly Insulin-Responsive Isolated Rat Heart Muscle Cells Yielded By a Modified Isolation Method, Life Sci 49, 1679, 1991 |
| 大鼠 | 大鼠, neonatal, 1-4 days | Cardiomyocytes | Collagenase Type 2: 0.12% | Krebs-Henseleit Buffer, CF | Marino, T.A., Walter, R.,Cobb, E., Palasiuk, M., Parsons, T., and Mercer, W.E.: Effects of Norepinephrine on Neonatal Rat Cardiocyte Growth and Differentiation, In Vitro Cell Dev Biol 26, 229, 1990 |
| 大鼠 | 大鼠 | Heart | Trypsin: 0.06% | 25mM HEPES buffered Locke's solution, CMF | Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and B綿羊 Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990 |
| (also b綿羊) | Adrenal chromaffin | ||||
| Paraneurons | |||||
| 大鼠 | 大鼠, adult, female | Myocytes | Collagenase Type 2: 0.05% | Joklik's MEM | Nag, A.C., Lee, M., and Kosiur, J.R.: Adult Cardiac Muscle Cells in Long-Term Serum-Free Culture:Myofibrillar Organization and Expression of Myosin Heavy Chain Isoforms, In Vitro Cell Dev Biol 26, 464, 1990 |
| 大鼠 | 大鼠s, AW, late-gestation pregnant females | Ventricular | Collagenase Type 2: 0.05 - 0.08% | See reference | Engelmann, G., McTiernan, C., Gerrity, R., and Samarel, A.: Technique 2 (6), 279, 1990 |
| Cardiomyocytes | |||||
| 大鼠 | 大鼠,SD, albino, 10-14 weeks | Myocytes | Collagenase Type 1: 100 u/ml and 150 u/ml | Krebs Ringer bicarbonate buffer | Berg, I., Guse, A.H., and Gercken, G.: Carbamoylcholine-Induced Accumulation of Inositol Mono-,Bis-,Tris-, And Tetrakisphosphates In Isolated Cardiac Myocytes From Adult Rats, Biochim Biophys Acta 1010, 100, 1989 |
| 大鼠 | 大鼠, SD, adult, female, 175 - 225 g | Myocytes | Collagenase: 0.1% | Joklik's medium | Bugaisky, L.B. and Zak, R.: Differentiation of Adult Rat Cardiac Myocytes in Cell Culture, Circ Res 64, 493, 1989 |
| 大鼠 | 大鼠, SD, male, 250 - 350 g | Myocytes, ventricular | Collagenase Type 1: 90 and 100 u/ml | Joklik's solution with and without calcium | De Young, M., Giannattasio, B., and Scarpa, A.: Isolation of Calcium-Tolerant Atrial And Ventricular Myocytes From Adult Rat Heart,Methods Enzymology Vol. 173,, , 662, 1989 |
| 大鼠 | 大鼠, SD, 2-4 day old | Cardiomyocytes | Trypsin: 0.1% | HBSS | Toraason, M., Luken, M., Breitenstein, M., Krueger, J., and Biagini, R.:Comparative Toxicity of Allylamine and Acrolein in Cultured Myocytes and Fibroblasts from Neonatal Rat Heart, Toxicology 56, 107, 1989 |
| Fibroblasts | |||||
| 大鼠 | 大鼠, SD, male | Aortic smooth muscle | Trypsin: 0.05% | DMEM | Cornwell, T., and Lincoln, T.: Regulation of Intracellular Ca2+ Levels in Cultured Vascular Smooth Muscle Cells, J Biol Chem 264 (2), 1146, 1989 |
| 大鼠 | 大鼠, adult, 150-200 g | Myocytes, atria | Collagenase Type 1: 100 u/ml | Kreb's Ringer bicarbonate - HEPES buffer | McMahon, K.K.: A Study of Adult Rat Atrial Myocyte Attachment to Extricellular Matrix Components and Long Term Culture,Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, Eds), Elsevier Science Publishing Co. Inc., 318, 1988 |
| 大鼠 | SD大鼠 | Myocytes | Collagenase Type 2: 0.1% | Kreb's Ringer w/ calcium | Buxton, I.L.O. and Doggwiler, K.O.: Alpha1-Adrenergic Receptor Signal Transduction in the Adult Rat Cardiac Myocyte,Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, eds), , 298, 1988 |
| 大鼠 | SD大鼠, 雄性, 250-350 克 | Myocytes, heart | Hyaluronidase: 0.03% | Bicarbonate-buffered medium | Kim, D. and Smith, T.W.: Temporal Variation in Contractile State And [Ca++] in Isolated Adult Rat And Guinea Pig Cardiac Myocytes,Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, eds), Elsevier Science Publishing Co. Inc., 370, 1988 |
| 大鼠 | Wistar大鼠, 2-4 天neonates | Myoblast, cardiac | Trypsin: 0.1% | Standardized Medium | Grynberg, A., Degois, M., Guenot, L., and Athias, P.: Primary Rat Cardiac Cell Culture: Diet of the Mother Rats as a Determinant Parameter of Cardiomyoblast Production from Neonates, Biol Cell 57, 89, 1986 |
| (see reference) | |||||
| 大鼠 | 大鼠, SD, 懷孕的 | Myocytes | Trypsin: 2.4 u/ml | DMEM | Freerksen, D., Schroedl, N., and Hartzell, C.: Control of Enzyme Activity Levels by Serum and Hydrocortisone in Neonatal Rat Heart Cells Cultured in Serum-Free Medium, J Cell Physiol 120, 126, 1984 |
| 大鼠 | 大鼠, SD, 雌性, 12 周 | Myocytes | Collagenase Type 1: 0.06% | Krebs Ringer bicarbonate buffer | Piper, H.M., Probst, I., Schwartz, P., Hutter, F.J., and Spieckermann, P.G.:Culturing of Calcium Stable Adult Cardiac Myocytes, J Mol Cell Cardiol 14, 397, 1982 |
| 大鼠 | 大鼠, 雄性/雌性, 200-250 克 | Muscle | Hyaluronidase: 0.1% | Krebs Ringer phosphate buffer, CMF | Nag, A. and Cheng, M.: Adult Mammalian Cardiac Muscle Cells in Culture,Tissue Cell 13, 515, 1981 |
| 大鼠 | 大鼠, 雌性, 成年, 200 - 300 克 | Myocytes | Collagenase Type 2: 0.1% | Joklik's MEM | Claycomb, W.C., and Palazzo, M.C.: Culture of The Terminally Differentiated Adult Cardiac Muscle Cell: A Light and Scanning Electron Microscope Study,Dev Biol 80, 466, 1980 |
| 大鼠 | SD大鼠, 成年, 雄性, 200-250 克 | Myocytes | Hyaluronidase: 0.10% | Joklik MEM, CF | Frangakis, C., Bahl, J., McDaniel, H., and Bressler, R.: Tolerance to Physiological Calcium by Isolated Mycocytes from the Adult Rat Heart; An Improved Cellular Preparation, Life Sci 27, 815, 1980 |
| 大鼠 | SD大鼠, 雌性, 12 周, 250 克 | Myocytes | Collagenase Type 1: 0.20% | Kreb's Ringer bicarbonate buffer | Powell, T., Terrar, D.A., and Twist, V.W.: Electrical Properties of Individual Cells Isolated From Adult Rat Ventricular Myocardium, J Physiol 302, 131, 1980 |
| Ventricular myocardium | |||||
| 大鼠 | 大鼠, 10 周 | Myocytes | Collagenase Type 1: 0.1% | Saline | Bishop, S., Oparil, S., Reynolds, R., and Drummond, J.: Regional Myocyte Size in Normotensive and Spontaneously Hypertensive Rats, Hypertension 1 (4), 378, 1979 |
| 大鼠 | 大鼠, 雄性, 200-300 克 | Myocytes | Hyaluronidase: 82 u/ml | Kreb's Henseleit buffer | Farmer, B., Harris, R., Jolly, W., Hathaway, D., Katzberg, A., Watanabe, A., Whitlow, A., and Besch, H.: Isolation and Characterization of Adult Rat Heart Cells, Arch Biochem Biophys 179, 545, 1977 |
| 大鼠 | 大鼠,雌性, 白化變種, 200-250克 | Myocytes | Hyaluronidase: 0.1% | Kreb's Ringers phosphate | Nag, A., Fischman, D., Aumont, M., and Zak, R.: Studies of Isolated Adult Rat Heart Cells: The Surface Morphology and the Influence of Extracellular Calcium Ion Concentration on Cellular Viability, Tissue Cell 9 (3), 419, 1977 |
| 大鼠 | 大鼠, 成年, 雄性, 150-200克 | Myocytes | Collagenase: 0.1% | MEM CF | Grosso, D., Frangakis, C., Carlson, E., and Bressler, R.: Isolation and Characterization of Myocytes From the Adult Rat Heart, Prep Biochem 7 (5), 383, 1977 |
| Hyaluronidase: 0.1% | |||||
| 大鼠 | 大鼠, 成年的 | Myocytes | Collagenase: 0.1% | Perfusing solution | Moustafa, E., Skomedal, T., Osnes, J., and Oye, I.: Cyclic Amp Formation and Morphology of Myocardial Cells Isolated from Adult Heart: Effect of Ca2+ and Mg2+, Biochim Biophys Acta 421, 411, 1976 |
| 大鼠 | SD大鼠, 白化變種, 雄性, 180-230 克 | Heart ventricles, beating | Collagenase: 0.05-0.2% | Phosphate buffer | Glick, M., Burns, A., and Reddy, W.: Dispersion and Isolation of Beating Cells from Adult Rat Heart, Anal Biochem 61, 32, 1974 |
| 大鼠 | 大鼠, 希伯來聯(lián)合國, 3 & 17 個月, 150-210 克 | Myocytes | Trypsin: 0.05% | Versene buffer | Bierman, E.L., Stein, O., and Stein, Y.: Lipoprotein Uptake and Metabolism by Rat Aortic Smooth Muscle Cells in Tissue Culture, Circ Res 35, 136, 1974 |
| 大鼠 | 大鼠, 2-5 天 | Myocytes | Trypsin: 0.01% | Saline A | Speicher, D., and McCarl, R.: Pancreatic Enzyme Requirements for the Dissociation of Rat Hearts for Culture, In Vitro 10, 30, 1974 |
| 大鼠 | 大鼠, SD, male, 150-200克 | Myocytes | Hyaluronidase: 0.05% | (see reference) | Pretlow II, T., Glick, M., and Reddy, W.: Separation of Beating Cardiac Myocytes from Suspensions of Heart Cells, Am J Pathol 67 (2), 215, 1972 |
| 大鼠 | 大鼠, albino, male, 250 克 | Myocytes | Hyaluronidase: 0.20% | Hank's solution, CF | Berry, M., Friend, D., and Scheuer, J.: Morphology and Metabolism of Intact Muscle Cells Isolated from Adult Rat Heart, Circ Res 26, 679, 1970 |
| 大鼠 | 大鼠, 成年的, 雄性, 250 克 | Myocytes | Trypsin: 0.1% | Saline A | Vahouny, G.V., Wei, R., Starkweather, R., and Davis, C.: Preparation of Beating Heart Cells From Adult Rats, Science 167, 1616, 1970 |
| 大鼠 | Wistar大鼠, 1-4 天大 | Myocytes | Trypsin: 0.125% | Gey's BSS | Mark, G., and Strasser, F.: Pacemaker Activity and Mitosis in Cultures of Newborn Rat Heart Ventricle Cells, Exp Cell Res 44, 217, 1966 |
| 貝類動物 | 太平洋牡蠣 | Haemocytes | Trypsin: | L15 medium | Le Duff, R., Lipart, C., and Renault, T.: Primary Culture of Pacific Oyster, Crassostrea gigas, heart cells, J Tiss Cul Meth 16, 67, 1994 |
| 貝類動物 | 蚌, 3.0 - 4.5 厘米 | Myocytes | Collagenase Type 1: 2% | L15 medium | Wen, C., Kou, G., and Chen, S.: C*tion of Cells From the Heart of the Hard Clam, Merretrix lusoria (Roding), J Tiss Cul Meth 15, 123, 1993 |
| Fibroblasts |
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